Our NPM1/MLF1 fusion probe is designed to detect NPM1/MLF1 fusions. The probe comes labeled in orange and green, but can be customized to meet your needs.
Gene Background: The most common rearrangements in acute myeloid leukemia (AML) with myelodysplasia-related changes involve the 5q31-q35 region, including t(3;5)(q25;q35). This particular translocation results in NPM1/MLF1 fusion, producing a chimeric gene that includes roughly have of NPM1 fused to nearly all of MLF1. NPM1/MLF1’s contribution to malignant transformation remains unclear. However, because MLF1 is not normally expressed in hematopoietic tissue, the fusion may promote ectopic MLF1 expression in hematopoietic cells, leading to leukemogenesis. Haploinsufficiency of wild-type NPM1 may also play a role. The fusion is found in approximately 0.5% of AML cases, where it's more frequent in younger patients.
Source: Aypar U, et al. (2014) Journ Molec Diagnostics. 527-532.
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
Gene Summary
The protein encoded by this gene is involved in several cellular processes, including centrosome duplication, protein chaperoning, and cell proliferation. The encoded phosphoprotein shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning ribosomal proteins and core histones from the nucleus to the cytoplasm. This protein is also known to sequester the tumor suppressor ARF in the nucleolus, protecting it from degradation until it is needed. Mutations in this gene are associated with acute myeloid leukemia. Dozens of pseudogenes of this gene have been identified. [provided by RefSeq, Aug 2017]
Gene Details
Gene Symbol: NPM1
Gene Name: Nucleophosmin 1
Chromosome: CHR5: 170814707-170837888
Locus: 5q35.1